Methanol-Associated Matrix Effects in Electrospray Ionization Tandem Mass Spectrometry

Methanol-associated matrix effects in electrospray ionization tandem mass spectrometry.

BACKGROUND Matrix effects can profoundly reduce the performance of electrospray ionization mass spectrometry. Preliminary observations indicated that the methanol used in the mobile phase could be a source of differential ionization or ion suppression. METHODS Drug stability studies, analysis of biological extracts, mixing experiments, and postcolumn infusions were used to test 9 commercial m...

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1. Sykes S, ed. Electromagnetic compatibility for medical devices: issues and solutions. Food and Drug Administration/Association for the Advancement of Medical Instrumentation (FDA/ AAMI) Conference; 1995 May 24–25; Anaheim, CA. Arlington (VA): Association for the Advancement of Medical Instrumentation; 1996. 2. Memorandum: electromagnetic compatibility for medical devices: issues and solution...

chromatography–electrospray ionization tandem mass spectrometry

Isoelectric Focusing, Matrix-assisted Laser Desorption/ionization Time-of- Flight Mass Spectrometry, Electrospray Ionization Mass Spectrometry

LEVEL: INTERMEDIATE D iscovery, development, and commercialization of novel biologics frequently involve collaboration between two or more companies. In the context of these business relationships, transfer of technology from one institution to another is a crucial step that needs to be executed f lawlessly and rapidly. Follow-up activities usually include the development of productive, reliabl...

Electrospray and tandem mass spectrometry in biochemistry.

Over the last 20 years, biological MS has changed out of all recognition. This is primarily due to the development in the 1980s of 'soft ionization' methods that permit the ionization and vaporization of large, polar, and thermally labile biomolecules. These developments in ionization mode have driven the design and manufacture of smaller and cheaper mass analysers, making the mass spectrometer...